A validated chiral LC method for enantiomeric separation of intermediate of lopinavir by using cellulose based chiral stationary phase

2R-(1-Tetrahydro pyrimid-2-onyl)-3-methyl butanoic acid. (STHPA) (Figure 1) is a intermediate for Lopinavir (Figure 2). Lopinavir is antiretroviral drugs acting as inhibitors of human immunodeficiency virus (HIV) protease, a class of drugs that has markedly improved morbidity and mortality of HIV infected patients[1]. Within the highly active antiretroviral therapy (HAART) they are used in a fixed combination (33 mg ritonavir + 133 mg lopinavir) called Kaletra with ritonavir added to boost lopinavir by inhibition of cytochrome P450 (CYP) CYP3A isozymes and active transport by P-glycoprotein[2,3]. Even though HAART has markedly improved the clinical outcome of HIVP.Surya Prakash Rao1,2*, A.Nageswari2, V.Ranga Reddy1, Mukkanti2 Integrated Product Development, Dr. Reddy’s Laboratories Limited, Bachupally, Qutubullapur, Rangareddy District 500 072, Andhra Pradesh, (INDIA) Department of Chemistry, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 072, Andhra Pradesh, (INDIA) E-mail: [email protected]; [email protected] Received: 16 June, 2011 ; Accepted: 16 July, 2011